Microbiology Services

Microbiology

All microbiology submissions are referred to our specialist Microbiology Lab at our Knutton site [see www.capl.co.uk].

Our microbiology reports carry interpretive comment, when appropriate and if clinical history is provided, and, because microbiology testing can be run in conjunction with our well-established diagnostic histopathology and cytology service, the true significance of the isolates can often be determined.

Submissions for microbiology are considered on a case-by case-basis, applying the principles of systematic microbiology, wherein cultures for those particular pathogens that may be associated with a specific site or clinical presentation are routinely sought at no additional cost to our clients. We believe this provides the most comprehensive and cost-effective service available.

 

What do we offer?

 

Bacterial Culture & Sensitivity

Routine culture of swabs, tissues, fluids, washes, etc. for ALL organisms of veterinary importance, including the higher bacteria (Actinomyces, Nocardia, Streptomyces and Mycobacteria), Mycoplasma, dermatophyte and other fungi (e.g. Aspergillus spp.), anaerobes, lipophilic and lipid-dependent Malassezia spp., etc.
In a great number of instances (depending upon the site sampled and the history provided) culture for these additional organisms is included in the initial culture and sensitivity request, thereby incurring no additional charge.

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1) Meticillin resistance screening in staph. isolates showing two putative MRSA (blue) and one MRSP (white).

 

2) and 3) Positive D-test in a strain of Staphylococcus aureus, and a negative D-test in a strain of Staphylococcus pseudintermedius, both isolated from a tissue biopsy from the same animal (canine). This test detects the presence of a gene encoding for inducible resistance to clindamycin, and is performed on all staphylococcal isolates. A positive result indicates clindamycin should not be used for in-vivo therapy, despite apparent in-vitro susceptibility to that agent, as there would be rapid development of resistance upon exposure.

 

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4) Trichophyton mentagrophytes (obverse and reverse of colonies), a dermatophyte (‘ringworm’) fungus.

 

5) and 6) ESBL screening in multi-resistant Escherichia coli.

 

5) ESBL-negative strain 

 

6) CTX-M type ESBL-producing strain. Detection is based on clavulanate augmentation of susceptibility to third and fourth generation cephalosporins.

 

All isolates are routinely screened to determine whether they may be multi-antimicrobial resistant bacteria, such as meticillin-resistant staphylococci (of all species, including MRSA and MRSP), Extended Spectrum Beta Lactamase (ESBL)-producing coliforms, Vancomycin-resistant enterococci, carbapenamase-producing coliforms or Pseudomonas, etc.

We are involved in several surveillance schemes regarding the development and spread of multi-drug resistant organisms, and are actively engaged in a number of studies, the findings of which are regularly presented at scientific meetings, or published in the scientific press.

 

Fungal culture
Cultures for dermatophyte fungi and yeasts, and also for saprophytic fungi such as Aspergillus.

 

Full urinalysis

Full urinalysis includes culture (including direct sensitivity testing), sediment exam, USG and dipstick analysis
We also offer urine cytology, calculus analysis (by IR spectroscopy), etc.

 

Full faecal analysis

Our routine faecal analysis includes McMasters for ova and cysts, examination for Giardia and culture for Salmonella, Campylobacter, Yersinia and E.coli O157/O157:H7). We also offer Baermanns examination for Metastronglye larvae, Angiostrongylus vasorum serology and toxin testing for Clostridium perfringens and difficile.

Please feel free to contact the Knutton site to discuss your specific testing requirements or to request a more comprehensive price list for the microbiology and other clinical pathology testing available.